phosphorylated stat3 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
phosphorylated stat3
Phosphorylated Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pathscan+total+stat3+sandwich+elisa+kit/PathScan+Total+Smad2%2F3+Sandwich+ELISA+Kit/pmc13032648-151-9-15
Average 96 stars, based on 180 article reviews
Phosphorylated Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pathscan+total+stat3+sandwich+elisa+kit/PathScan+Total+Smad2%2F3+Sandwich+ELISA+Kit/pmc13032648-151-9-15
Average 96 stars, based on 180 article reviews
phosphorylated stat3 - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata"
Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata
Journal: Journal of Nanobiotechnology
doi: 10.1186/s12951-026-04214-7
Figure Legend Snippet: ( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting STAT3 mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)
Techniques Used: Isolation, Staining, Fluorescence, Expressing, Luciferase, Reporter Assay
Figure Legend Snippet: ( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)
Techniques Used: Expressing, Transfection, Wound Healing Assay, Transwell Assay, Ex Vivo
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Sandwich ELISA:Article Title: Lopinavir Derivative as Potent P‑gp Inhibitor Enables Delivery through HPMA Copolymer Conjugates and Overcoming Tumor Chemoresistance to Conventional Cytostatic Drugs Article Snippet: .. The experiment was performed using a Article Title: Ligand-mediated PAI-1 inhibition in a mouse model of peritoneal carcinomatosis Article Snippet: Levels of PAI-1 (DSE100), IL-6 (D6050), IL-10 (D1000B), CCL-2 (DCP00), and MMP-9 (DMP900) in CFA were measured using human Quantikine ELISA kits (R&D Systems). .. Total STAT3 and p-STAT3 (Tyr705) were measured by Article Title: Spatial Heterogeneity, Stromal Phenotypes, and Therapeutic Vulnerabilities in Colorectal Cancer Peritoneal Metastasis Article Snippet: The harvested cells were lysed with lysis buffer (Cell Signaling Technology, 9803S) in the presence of protease and phosphatase inhibitors (Thermo Fisher Scientific, 1861281), followed by total protein concentration measured by Bradford protein assay (Bio-Rad, 5000006). .. Then 25 μg of the protein lysate was used to measure total STAT3 and p-STAT3 (Tyr705) by ELISA using the Article Title: Lopinavir Derivative as Potent P-gp Inhibitor Enables Delivery through HPMA Copolymer Conjugates and Overcoming Tumor Chemoresistance to Conventional Cytostatic Drugs. Article Snippet: .. The experiment was performed using a Article Title: Chemosensitization of tumors via simultaneous delivery of STAT3 inhibitor and doxorubicin through HPMA copolymer-based nanotherapeutics with pH-sensitive activation. Article Snippet: .. Determination of STAT3 and pSTAT3 levels in cell lysates (50 μg of protein) was carried out using a Article Title: Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling. Article Snippet: Drug repurposing is a promising strategy for identifying new applications for approved drugs.. Here, we describe a polymer biomaterial composed of the antiretroviral drug ritonavir derivative (5-methyl-4-oxohexanoic acid ritonavir ester; RD), covalently bound to HPMA copolymer carrier via a pH-sensitive hydrazone bond (P-RD).. Apart from being more potent inhibitor of P-glycoprotein in comparison to ritonavir, we found RD to have considerable cytostatic activity in six mice (IC50 ~ 2.3–17.4 μM) and six human (IC50 ~ 4.3–8.7 μM) cancer cell lines, and that RD inhibits the migration and invasiveness of cancer cells in vitro. Enzyme-linked Immunosorbent Assay:Article Title: Spatial Heterogeneity, Stromal Phenotypes, and Therapeutic Vulnerabilities in Colorectal Cancer Peritoneal Metastasis Article Snippet: The harvested cells were lysed with lysis buffer (Cell Signaling Technology, 9803S) in the presence of protease and phosphatase inhibitors (Thermo Fisher Scientific, 1861281), followed by total protein concentration measured by Bradford protein assay (Bio-Rad, 5000006). .. Then 25 μg of the protein lysate was used to measure total STAT3 and p-STAT3 (Tyr705) by ELISA using the |
