phosphorylated stat3  (Cell Signaling Technology Inc)

Journal: Journal of Nanobiotechnology

Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

doi: 10.1186/s12951-026-04214-7

Figure Lengend Snippet: ( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting STAT3 mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)

Article Snippet: After transfer to polyvinylidene difluoride membranes, rabbit antibodies against phosphorylated STAT3 ( p -STAT3) (1∶2000, CST), mouse antibody against stat3 (1∶2000, CST), mouse antibody against β-actin (1∶1000, Beyotime), and mouse antibody against STAT3 (1∶1000, Beyotime) were used.

Techniques: Isolation, Staining, Fluorescence, Expressing, Luciferase, Reporter Assay

Journal: Journal of Nanobiotechnology

Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

doi: 10.1186/s12951-026-04214-7

Figure Lengend Snippet: ( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: After transfer to polyvinylidene difluoride membranes, rabbit antibodies against phosphorylated STAT3 ( p -STAT3) (1∶2000, CST), mouse antibody against stat3 (1∶2000, CST), mouse antibody against β-actin (1∶1000, Beyotime), and mouse antibody against STAT3 (1∶1000, Beyotime) were used.

Techniques: Expressing, Transfection, Wound Healing Assay, Transwell Assay, Ex Vivo

Journal: ChemistryOpen

Article Title: Synthesis of compounds based on the active domain of cabotegravir and their application in inhibiting tumor cells activity

doi: 10.1002/open.202300284

Figure Lengend Snippet: (a): Western blot was used to detect the protein expression of KYSE30 cells treated with different concentrations of compounds. (b): Western blot results showed that the expression of Stat3 protein was significantly decreased after treatment with different concentrations of the compounds. (c): The expression of Smad2/3 protein was significantly decreased after treatment with different concentrations of drugs. (d): The expression of cleaved caspase3 protein was significantly increased after treatment with different drug concentrations. Data are shown as the mean±SD and were analyzed with one‐way ANOVA. (*P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001, compared with the control group).

Article Snippet: After washing with TBST solution, the membrane was incubated overnight at 4 °C with the primary antibody Stat3 (1 : 2000, CST), Smad2/3 (1 : 1000, CST), GAPDH (1 : 5000, Sangon Biotech), and cleaved caspase 3 (1 : 1000, CST).

Techniques: Western Blot, Expressing, Control